Primary Identifier | MGI:2683764 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Blm |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A floxed PGK-neomycin resistance gene was inserted into intron 8 and an additional loxP site was placed in intron 7, 75 bp from the start of exon 8. Both exon 8 and the neomycin gene were subsequently removed from correctly targeted ES cells by Cre-mediated recombination. A frame shift mutation is predicted at the splice site between exon 7 and exon 9 in the resulting transcript. |