| Primary Identifier | MGI:6885589 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | Dnhd1 |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | CRISPR/Cas9 technology using sgRNA 5'-CTAGGGAAAATAATTTAGATAGG-3' generated a 31-bp deletion in exon 22 resulting in a truncated protein c.5572_5602del (p.1858Trpfs*24). Reverse-transcription quantitative PCR confirmed absence of mRNA expression in the testes. |
