| Primary Identifier | MGI:5796264 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Fan1 |
| Transmission | Germline | Strain of Origin | C57BL/6N-A<tm1Brd> |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | KOMP-CSD |
| molecularNote | The L1L2_Bact_P cassette was inserted at position 64018509 of Chromosome 7 upstream of the critical exon 3 (Build GRCm39). The cassette is composed of an FRT site followed by lacZ sequence and a loxP site. This first loxP site is followed by neomycin resistance gene under the control of the human beta-actin promoter, SV40 polyA, a second FRT site and a second loxP site. A third loxP site is inserted downstream of the targeted exon 4 at position 64021818. The critical exons 3 an 4 is/are thus flanked by loxP sites. Flp recombinase expression followed by subsequent cre expression resulted in a knockout allele in which the lacZ sequence, neomycin selecton cassette and exons 3 and 4 are deleted. |
