| First Author | Sun-Wada GH | Year | 2009 |
| Journal | J Cell Sci | Volume | 122 |
| Issue | Pt 14 | Pages | 2504-13 |
| PubMed ID | 19549681 | Mgi Jnum | J:150327 |
| Mgi Id | MGI:3850356 | Doi | 10.1242/jcs.050443 |
| Citation | Sun-Wada GH, et al. (2009) Direct recruitment of H+-ATPase from lysosomes for phagosomal acidification. J Cell Sci 122(Pt 14):2504-13 |
| abstractText | The nascent phagosome progressively establishes an acidic milieu by acquiring a proton pump, the vacuolar-type ATPase (V-ATPase). However, the origin of phagosomal V-ATPase remains poorly understood. We found that phagosomes were enriched with the V-ATPase a3 subunit, which also accumulated in late endosomes and lysosomes. We modified the mouse Tcirg1 locus encoding subunit a3, to express an a3-GFP fusion protein. Live-cell imaging and immunofluorescence microscopy revealed that nascent phagosomes received the a3-GFP from tubular structures extending from lysosomes located in the perinuclear region. Macrophages from a3-deficient mice exhibited impaired acidification of phagosomes and delayed digestion of bacteria. These results show that lysosomal V-ATPase is recruited directly to the phagosomes via tubular lysosomes to establish the acidic environment hostile to pathogens. |
