| First Author | Koay HF | Year | 2019 |
| Journal | Nat Commun | Volume | 10 |
| Issue | 1 | Pages | 2243 |
| PubMed ID | 31113973 | Mgi Jnum | J:276912 |
| Mgi Id | MGI:6323707 | Doi | 10.1038/s41467-019-10198-w |
| Citation | Koay HF, et al. (2019) Diverse MR1-restricted T cells in mice and humans. Nat Commun 10(1):2243 |
| abstractText | Mucosal-associated invariant T (MAIT) cells express an invariant TRAV1/TRAJ33 TCR-alpha chain and are restricted to the MHC-I-like molecule, MR1. Whether MAIT cell development depends on this invariant TCR-alpha chain is unclear. Here we generate Traj33-deficient mice and show that they are highly depleted of MAIT cells; however, a residual population remains and can respond to exogenous antigen in vitro or pulmonary Legionella challenge in vivo. These residual cells include some that express Trav1(+) TCRs with conservative Traj-gene substitutions, and others that express Trav1(-) TCRs with a broad range of Traj genes. We further report that human TRAV1-2(-) MR1-restricted T cells contain both MAIT-like and non-MAIT-like cells, as judged by their TCR repertoire, antigen reactivity and phenotypic features. These include a MAIT-like population that expresses a public, canonical TRAV36(+) TRBV28(+) TCR. Our findings highlight the TCR diversity and the resulting potential impact on antigen recognition by MR1-restricted T cells. |
