| First Author | Pan YX | Year | 1998 |
| Journal | J Neurochem | Volume | 70 |
| Issue | 6 | Pages | 2279-85 |
| PubMed ID | 9603192 | Mgi Jnum | J:47688 |
| Mgi Id | MGI:1203928 | Doi | 10.1046/j.1471-4159.1998.70062279.x |
| Citation | Pan YX, et al. (1998) Cloning and characterization of a mouse sigma1 receptor. J Neurochem 70(6):2279-85 |
| abstractText | A cDNA clone (S2-1a) isolated from a mouse brain cDNA library, using a guinea pig sigma(1) cDNA as probe, has high homology to the predicted protein sequence of the guinea pig (88%) and human (90%) sigma(1) receptors. Northern analysis revealed a major mRNA of similar to 1.8 kb in a wide range of mouse tissues, with highest levels in brain, liver, kidney, and thymus. Southern analysis and chromosomal mapping in the mouse suggested a single-copy gene in region A5-B2 of chromosome 4. Expression of the clone in MCF-7 and CHO cells led to a pronounced increase in (+)-[H-3] pentazocine binding with a selectivity profile consistent with sigma(1) receptors. In vitro translation yielded a protein of similar to 28 kDa, as did transfection of a probe containing the hemagglutinin (HA) epitope (S2-1a.HA) into CHO cells, as determined by western analysis using an antibody directed against HA. (+)-[H-3]- Pentazocine binding to immunopurified HA-tagged receptor demonstrated conclusively that S2-1a.HA encodes a high-affinity (+)-[3H]pentazocine binding site with characteristics of a murine sigma(1) receptor. An antisense oligodeoxynucleotide designed from S2-1a potentiated opioid analgesia in vivo. |
