| First Author | Dube PR | Year | 2018 |
| Journal | Atherosclerosis | Volume | 270 |
| Pages | 199-204 | PubMed ID | 29290366 |
| Mgi Jnum | J:301840 | Mgi Id | MGI:6507253 |
| Doi | 10.1016/j.atherosclerosis.2017.12.025 | Citation | Dube PR, et al. (2018) Reduced calcification and osteogenic features in advanced atherosclerotic plaques of mice with macrophage-specific loss of TRPC3. Atherosclerosis 270:199-204 |
| abstractText | BACKGROUND AND AIMS: Recent in vitro studies have showed that in macrophages, deletion of the non-selective Ca(2+)-permeable channel TRPC3 impairs expression of the osteogenic protein BMP-2. The pathophysiological relevance of this effect in atherosclerotic plaque calcification remains to be determined. METHODS: We used Ldlr(-/-) mice with macrophage-specific loss of TRPC3 (MacTrpc3(-/-)/Ldlr(-/-)) to examine the effect of macrophage Trpc3 on plaque calcification and osteogenic features in advanced atherosclerosis. RESULTS: After 25 weeks on high fat diet, aortic root plaques in MacTrpc3(-/-)/Ldlr(-/-) mice showed reduced size, lipid and macrophage content compared to controls. Plaque calcification was decreased in MacTrpc3(-/-)/Ldlr(-/-) mice, and this was accompanied by marked reduction in BMP-2, Runx-2 and phospho-SMAD1/5 contents within macrophage-rich areas. Expression of Bmp-2 and Runx-2 was also reduced in bone marrow-derived macrophages from MacTrpc3(-/-)/Ldlr(-/-) mice. CONCLUSIONS: These findings show that, in advanced atherosclerosis, selective deletion of TRPC3 in macrophages favors plaque regression and impairs the activity of a novel macrophage-associated, BMP-2-dependent mechanism of calcification. |
