| First Author | Hatada I | Year | 1995 |
| Journal | Nucleic Acids Res | Volume | 23 |
| Issue | 1 | Pages | 36-41 |
| PubMed ID | 7870588 | Mgi Jnum | J:22780 |
| Mgi Id | MGI:70668 | Doi | 10.1093/nar/23.1.36 |
| Citation | Hatada I, et al. (1995) Allele-specific methylation and expression of an imprinted U2af1-rs1 (SP2) gene. Nucleic Acids Res 23(1):36-41 |
| abstractText | The mouse U2af1-rs1(SP2) gene, which was cloned by a two-dimensional genome scanning method, is expressed exclusively from the paternally inherited chromosome. This gene has significant similarity to U2AF and located in chromosome 11, of which maternal duplication/paternal deficiency results in a small body. In this report, we cloned genomic U2af1-rs1(SP2) and found its promoter was methylated in a maternal-allele-specific manner. This allelic methylation was not established in parental gametes, but established between 1.5 d.p.c. and 12.5 d.p.c. on the contrary, the allele-specific expression occurred in the two-cell stage when transcription initiates. Absence of the methylation of the upstream region in this stage indicates that methylation is not necessary for inactivation of the expression. |
