| Primary Identifier | MGI:7410963 | Allele Type | Targeted |
| Attribute String | Conditional ready, Epitope tag, Inducible, Reporter, Transactivator | Gene | Igs7 |
| Transmission | Germline | Strain of Origin | (129S6/SvEvTac x C57BL/6NCrl)F1 |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell = made from Ai99 Flp-in mouse (derived from G4) |
| molecularNote | The transgenic construct contains HS4 insulator-Tet response element/promoter (pTRE2)-loxP-Stop-loxP-CaMP8s-WPRE-pA-HS4 insulator-pCAG-lox2272-Stop-lox2272-FLAG-IRES-tTA2-WPRE-pA cassette was introduced to the Igs7 intergenic region. GCaMP8s (sensitive) incorporates a hexa-histidine affinity tag, an endothelial nitric oxide synthase peptide (ENOSP; modified with N19T, S24I, and S26M), a circularly permutated green fluorescent protein (cpGFP; modified with Q88E), the rat calcium-binding protein calmodulin (CaM; modified with F286Y and Q315H), and LKI amino acid linkers that replace the RS20 (previously referred to as M13) linkers. |
