| First Author | Sadler KE | Year | 2020 |
| Journal | Elife | Volume | 9 |
| PubMed ID | 32729832 | Mgi Jnum | J:293145 |
| Mgi Id | MGI:6450811 | Doi | 10.7554/eLife.58625 |
| Citation | Sadler KE, et al. (2020) Keratinocytes contribute to normal cold and heat sensation. Elife 9:e58625 |
| abstractText | Keratinocytes are the most abundant cell type in the epidermis, the most superficial layer of skin. Historically, epidermal-innervating sensory neurons were thought to be the exclusive detectors and transmitters of environmental stimuli. However, recent work from our lab (Moehring et al., 2018) and others (Baumbauer et al., 2015) has demonstrated that keratinocytes are also critical for normal mechanotransduction and mechanically-evoked behavioral responses in mice. Here, we asked whether keratinocyte activity is also required for normal cold and heat sensation. Using calcium imaging, we determined that keratinocyte cold activity is conserved across mammalian species and requires the release of intracellular calcium through one or more unknown cold-sensitive proteins. Both epidermal cell optogenetic inhibition and interruption of ATP-P2X4 signaling reduced reflexive behavioral responses to cold and heat stimuli. Based on these data and our previous findings, keratinocyte purinergic signaling is a modality-conserved amplification system that is required for normal somatosensation in vivo. |
