| First Author | Ye Q | Year | 2022 |
| Journal | Development | Volume | 149 |
| Issue | 16 | PubMed ID | 35831953 |
| Mgi Jnum | J:327901 | Mgi Id | MGI:7333312 |
| Doi | 10.1242/dev.200539 | Citation | Ye Q, et al. (2022) Understanding the development of oral epithelial organs through single cell transcriptomic analysis. Development 149(16):dev200539 |
| abstractText | During craniofacial development, the oral epithelium begins as a morphologically homogeneous tissue that gives rise to locally complex structures, including the teeth, salivary glands and taste buds. How the epithelium is initially patterned and specified to generate diverse cell types remains largely unknown. To elucidate the genetic programs that direct the formation of distinct oral epithelial populations, we mapped the transcriptional landscape of embryonic day 12 mouse mandibular epithelia at single cell resolution. Our analysis identified key transcription factors and gene regulatory networks that define different epithelial cell types. By examining the spatiotemporal patterning process along the oral-aboral axis, our results propose a model in which the dental field is progressively confined to its position by the formation of the aboral epithelium anteriorly and the non-dental oral epithelium posteriorly. Using our data, we also identified Ntrk2 as a proliferation driver in the forming incisor, contributing to its invagination. Together, our results provide a detailed transcriptional atlas of the embryonic mandibular epithelium, and unveil new genetic markers and regulators that are present during the specification of various oral epithelial structures. |
