Primary Identifier | MGI:6294929 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Nucks1 |
Transmission | Germline | Strain of Origin | (129S2/SvPas x C57BL/6NSlc)F1-Tg(CAG-EGFP,Acr-EGFP)2Osb |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 4. The neo cassette was removed through subsequent flp-mediated recombination. Exon 4 was deleted through subsequent cre-mediated recombination. Western blot analysis showed an absent of expression in mouse embryonic fibroblasts, liver, and epididymal adipose tissues. |