| First Author | Goldstein JC | Year | 2005 |
| Journal | Cell Death Differ | Volume | 12 |
| Issue | 5 | Pages | 453-62 |
| PubMed ID | 15933725 | Mgi Jnum | J:111870 |
| Mgi Id | MGI:3654985 | Doi | 10.1038/sj.cdd.4401596 |
| Citation | Goldstein JC, et al. (2005) Cytochrome c is released in a single step during apoptosis. Cell Death Differ 12(5):453-62 |
| abstractText | Release of cytochrome c from mitochondria is a central event in apoptotic signaling. In this study, we utilized a cytochrome c fusion that binds fluorescent biarsenical ligands (cytochrome c-4CYS (cyt. c-4CYS)) as well as cytochrome c-green fluorescent protein (cyt. c-GFP) to measure its release from mitochondria in different cell types during apoptosis. In single cells, the kinetics of cyt. c-4CYS release was indistinguishable from that of cyt. c-GFP in apoptotic cells expressing both molecules. Lowering the temperature by 7 degrees C did not affect this corelease, but further separated cytochrome c release from the subsequent decrease in mitochondrial membrane potential (DeltaPsi(m)). Cyt. c-GFP rescued respiration in cells lacking endogenous cytochrome c, and the duration of cytochrome c release was approximately 5 min in a variety of cell types induced to die by various forms of cellular stress. In addition, we could observe no evidence of caspase-dependent amplification of cytochrome c release or changes in DeltaPsi(m) preceding the release of cyt. c-GFP. We conclude that there is a general mechanism responsible for cytochrome c release that proceeds in a single step that is independent of changes in DeltaPsi(m). |
