| First Author | Kang M | Year | 2001 |
| Journal | Biochim Biophys Acta | Volume | 1519 |
| Issue | 3 | Pages | 167-74 |
| PubMed ID | 11418182 | Mgi Jnum | J:70195 |
| Mgi Id | MGI:2136559 | Doi | 10.1016/s0167-4781(01)00231-7 |
| Citation | Kang M, et al. (2001) Nd1, a novel murine Kelch family protein, may play the role of a housekeeping gene. Biochim Biophys Acta 1519(3):167-74 |
| abstractText | The murine Nd1 gene encodes a novel Kelch family protein and expresses two forms of mRNA, long (Nd1-L) and short (Nd1-S), in various tissues. We characterized the genomic organization of the Nd1 gene, and found that Nd1-L and Nd1-S consist of 16 and nine exons respectively, and that exons I-VIII are shared between them. Three transcription initiation sites were identified in the 5'-flanking region and the most 3' side (+1) is likely to be a major one. Promoter analysis revealed that the region between positions -247 and -86 was sufficient for expression, and that two Sp1-binding sites and one NF-kappaB-binding site in the region were critical for promoter activity. Furthermore, the promoter region lacks a TATA and a CAAT box and has a highly GC-rich region with two important Sp1-binding sites. These characteristics of the Nd1 gene promoter are similar to the properties of housekeeping genes. |
