| First Author | Miller M | Year | 1992 |
| Journal | Leukemia | Volume | 6 |
| Issue | 1 | Pages | 18-28 |
| PubMed ID | 1736010 | Mgi Jnum | J:869 |
| Mgi Id | MGI:49402 | Citation | Miller M, et al. (1992) Transformation of murine pre-B-lymphocytes by v-erb-B: progression to growth factor independence and tumorigenicity. Leukemia 6(1):18-28 |
| abstractText | v-erb-B is the principal transforming gene of avian erythroblastosis virus, a replication defective retrovirus that transforms erythroid and fibroblast cells in vitro and causes erythroleukemia and fibrosarcoma in vivo. We have used a recombinant murine retrovirus, based on the truncated genome of Moloney murine leukemia virus and containing a chimeric gag-v-erb-B gene, to determine the murine hematopoietic cells transformed by v-erb-B. Infection of day 16.5 murine embryonal cells in liquid culture with this virus resulted in the outgrowth of foci of loosely to non-adherent hematopoietic cells which grew in close association with an adherent monolayer. After several weeks in culture a clonal population of transformed pre-B-lymphocytes emerged from this transformation initiated, though still growth factor dependent, population. These transformed cells were growth factor independent, and were tumorigenic in syngeneic mice. The results indicate that although v-erb-B can initiate transformation of murine hematopoietic cells, additional events are required for establishment of the fully transformed growth factor independent, tumorigenic phenotype. This v-erb-B induced progression from growth factor dependence to independence provides an in vitro model system for analysing events involved in the initiation and maintenance of leukemia. |
