| Primary Identifier | MGI:3640671 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(Myh6-EP300)21Csk |
| Strain of Origin | C57BL/6 | Is Recombinase | false |
| Is Wild Type | false |
| description | The original publication (J:110549) does not name or distinguish among three lines derived from independent transgenic founders. Kawamura et al. (J:110550) identify lines 21 and 39. |
| molecularNote | A full-length cDNA encoding human E1A binding protein p300 was ligated to the 5' end of a 5.5-kb fragment of mouse genomic DNA that includes the entire, promoter-containing intergenic region between the beta- (downstream) and alpha-cardiac myosin heavy chain genes. RT-PCR with transgene-specific primers detected mRNA expression in heart, but not in lung, liver or kidney. Immunoblot analysis detected 8-9-fold as much of the (endogenous plus transgene-encoded) protein in cardiac nuclear extracts from transgenic than from wild-type mice. |
