| First Author | Matsunami H | Year | 1993 |
| Journal | J Cell Sci | Volume | 106 ( Pt 1) |
| Pages | 401-9 | PubMed ID | 8270638 |
| Mgi Jnum | J:14830 | Mgi Id | MGI:62990 |
| Doi | 10.1242/jcs.106.1.401 | Citation | Matsunami H, et al. (1993) Cell binding specificity of mouse R-cadherin and chromosomal mapping of the gene. J Cell Sci 106(Pt 1):401-9 |
| abstractText | R-cadherin was originally identified as a chicken cadherin expressed by the retina. Here, we describe the identification of a mouse homologue of R-cadherin. We isolated mouse cDNAs encoding a cadherin with 94% identity in amino acid sequence to the chicken R-cadherin, and defined this molecule as mouse R-cadherin. L cells transfected with the mouse R-cadherin cDNA acquired a cadherin-mediated cell-cell adhesiveness as found for other cadherins. To examine the binding specificity of mouse R-cadherin, L cells expressing this cadherin (mRL) were mixed with L cells expressing chicken R-cadherin (cRL), mouse N-cadherin (mNL), mouse E-cadherin (mEL) and mouse P-cadherin (mPL). While mRL cells randomly intermixed with cRL cells, those cells aggregated separately from mEL or mPL cells. Mixing of mRL with mNL cells gave an intermediate result; that is, they formed both separate and chimeric aggregates, suggesting that R- and N-cadherin can interact with each other although each has a preference to bind to its own type. Similar properties were previously found for chicken R-cadherin. Thus, the cell binding specificity of R-cadherin is entirely conserved between the two species, suggesting a conserved role for this protein in morphogenesis. We also located the mouse R-cadherin gene to chromosome 2. |
