| First Author | Ma Q | Year | 2006 |
| Journal | Mol Reprod Dev | Volume | 73 |
| Issue | 9 | Pages | 1075-83 |
| PubMed ID | 16804880 | Mgi Jnum | J:113018 |
| Mgi Id | MGI:3664351 | Doi | 10.1002/mrd.20485 |
| Citation | Ma Q, et al. (2006) Molecular cloning and characterization of SRG-L, a novel mouse gene developmentally expressed in spermatogenic cells. Mol Reprod Dev 73(9):1075-83 |
| abstractText | Full-length cDNA of a novel mouse gene upregulated in late stages of spermatogenic cells was cloned from mouse testis using overlapping RT-PCR and RACE. The mRNA of the gene was expressed mainly in diplotene/pachytene spermatocytes, round and elongating spermatids. We named this gene as SRG-L (Spermatogenesis Related Gene expressed in late stages of spermatogenic cells, GenBank Accession No. AY352586). The tissue-specific analysis showed a higher expression level in testis and spleen. The gene is mapped on chromosome 8q33.1 and contains 18 exons. The full-length of cDNA is 2,843 bp with an open reading frame (ORF) of 2,625 bp that encodes a 104 kDa protein (874 amino acids) with a putative transmembrane region. The bioinformatics analysis revealed that the SRG-L has two conserved regions, transglutaminase-like homologues domain and D-serine dehydratase domain, rich phosphorylation sites and methylation sites. The SRG-L protein was detected in diplotene/pachytene spermatocytes and spermatids by immunohistochemical staining and Western blot. The results suggest that SRG-L may play definite roles regulating differentiation of germ cells during spermatogenesis, particularly during meiosis and spermiogenesis. |
