| First Author | Sun-Wada GH | Year | 2021 |
| Journal | Sci Rep | Volume | 11 |
| Issue | 1 | Pages | 19355 |
| PubMed ID | 34588579 | Mgi Jnum | J:312380 |
| Mgi Id | MGI:6788266 | Doi | 10.1038/s41598-021-98952-3 |
| Citation | Sun-Wada GH, et al. (2021) Vacuolar-type proton ATPase is required for maintenance of apicobasal polarity of embryonic visceral endoderm. Sci Rep 11(1):19355 |
| abstractText | The endocytic compartments keep their interior acidic through the inward flow of protons and anions from the cytosol. Acidification is mediated by a proton pump known as vacuolar-type ATPase (V-ATPase) and transporters conferring anion conductance to the organellar membrane. In this study, we analysed the phenotype of mouse embryos lacking the V-ATPase c-subunit. The mutant embryos differentiated embryonic epithelial tissues, primitive endoderm, epiblast, and extraembryonic ectoderm; however, the organisation of these epithelia was severely affected. The apical-basal polarity in the visceral endoderm layer was not properly established in the mutant embryos, resulting in abnormal epithelial morphology. Thus, the function of V-ATPase is imperative for the establishment and/or maintenance of epithelial cell polarity, which is required for early embryogenesis. |
