| First Author | Bailón-Plaza A | Year | 1999 |
| Journal | Bone | Volume | 24 |
| Issue | 3 | Pages | 211-6 |
| PubMed ID | 10071913 | Mgi Jnum | J:53771 |
| Mgi Id | MGI:1333396 | Doi | 10.1016/s8756-3282(98)00171-9 |
| Citation | Bailon-Plaza A, et al. (1999) BMP-5 deficiency alters chondrocytic activity in the mouse proximal tibial growth plate. Bone 24(3):211-6 |
| abstractText | The role of bone morphogenetic protein-5 (BMP-5) in regulating chondrocytic activity during endochondral ossification was examined in the mouse proximal tibial growth plate. Short ear mice homozygous for the SEA/Gn point mutation in the coding region for BMP-5 (King, JA et al. Dev Biol 166:112122; 1994) and heterozygous long ear littermates were examined at 5 and 9 weeks of age (n = 9/group, four groups). Animals were injected with oxytetracycline to estimate the rate of growth and with bromodeoxyuridine to identify proliferative chondrocytes. Age-related changes in chondrocytic stereological and kinetic parameters were compared by image analysis of 1-microm-thick growth plate sections. The number of proliferative chondrocytes did not vary with age in either genotype, but proliferative phase duration increased significantly (approximately 67%) with age in the long ear mice, whereas no change was detected in the short ear mice. The number of hypertrophic chondrocytes increased significantly (approximately 27%) in the short ears, whereas this number decreased significantly (approximately 40%) in the long ears. There was a small, but significant, increase in hypertrophic phase duration (approximately 45%) in short ear mice, but no change was detected in the long ears. These results indicate that BMP-5 deficiency prevents age-related decelerations in chondrocytic proliferation and initiation of hypertrophic differentiation, suggesting a role of BMP-5 in inhibiting these processes. |
