| Primary Identifier | MGI:7548797 | Allele Type | Targeted |
| Attribute String | Dominant negative, Inserted expressed sequence, Reporter | Gene | Hprt1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Mutant mice express a dominant negative version of mouse Foxf1 under the control of the CMV promoter. The chimeric gene was constructed by fusing the open reading frame of this transcriptional activator to a cDNA fragment harboring the strong transcriptional repression domain of the Drosophila engrailed protein. This coding region was followed by a fragment harboring an IRES-GFP sequence to allow visualization of misexpressing cells. The bicistronic transgene cassette was integrated in the ubiquitously expressed X-chromosomal Hypoxanthine guanine phosphoribosyl transferase (Hprt) locus. |
