| First Author | Sun H | Year | 2011 |
| Journal | PLoS One | Volume | 6 |
| Issue | 5 | Pages | e20502 |
| PubMed ID | 21655218 | Mgi Jnum | J:288380 |
| Mgi Id | MGI:6222191 | Doi | 10.1371/journal.pone.0020502 |
| Citation | Sun H, et al. (2011) Endogenous synthesis of n-3 polyunsaturated fatty acids in Fat-1 mice is associated with increased mammary gland and liver syndecan-1. PLoS One 6(5):e20502 |
| abstractText | Long chain n-3 PUFA have been shown to have chemopreventive properties against breast cancer through various mechanisms. One pathway, studied in human breast cancer cell lines, involves upregulation of the proteoglycan, syndecan-1 (SDC-1) by n-3 PUFA-enriched LDL. Using Fat-1 mice that are able to convert n-6 to n-3 PUFA, we tested whether SDC-1 level in vivo is elevated in mammary glands due to endogenously synthesized rather than LDL-derived n-3 PUFA. Female Fat-1 and wild type (wt) mice were fed an n-6 PUFA- enriched diet for 7 weeks. Fatty acid analysis of plasma lipoproteins showed that total n-6 PUFA reflected dietary intake similarly in both genotypes (VLDL, 36.2+/-2.2 and 40.9+/-3.9; LDL, 49.0+/-3.3 and 48.1+/-2.0; HDL, 54.6+/-1.2 and 58.2+/-1.3, mean +/- SEM percent of total fatty acids for Fat-1 and wt animals respectively). Lipoprotein percent n-3 PUFA was also similar between groups. However, phospholipids and triglycerides extracted from mammary and liver tissues demonstrated significantly higher n-3 PUFA and a corresponding decrease in the ratio n-6/n-3 PUFA in Fat-1 compared to wt mice. This was accompanied by higher SDC-1 in mammary glands and livers of Fat-1 mice, thus demonstrating that endogenously synthesized n-3 PUFA may upregulate SDC-1 in the presence of high dietary n-6 PUFA. |
