| First Author | Lee AC | Year | 2005 |
| Journal | Cell Cycle | Volume | 4 |
| Issue | 1 | Pages | 177-82 |
| PubMed ID | 15611643 | Mgi Jnum | J:157149 |
| Mgi Id | MGI:4430064 | Doi | 10.4161/cc.4.1.1354 |
| Citation | Lee AC, et al. (2005) Specific association of mouse MDC1/NFBD1 with NBS1 at sites of DNA-damage. Cell Cycle 4(1):177-82 |
| abstractText | Human MDC1/NFBD1 has been found to interact with key players of the DNA-damage response machinery. Here, we identify and describe a functional homologue of MDC1/ NFBD1 in Mus musculus. The mouse homologue, mMDC1, retains the key motifs identified in the human protein and in response to ionizing radiation forms foci that co-localize with the MRE11-RAD50-NBS1 (MRN) complex and factors such as gammaH2AX and 53BP1. In addition, mMDC1 is associated with DNA damage sites generated during meiotic recombination as well as the X and Y chromosomes during the late stages of meiotic prophase I. Finally, whereas MDC1 shows strong colocalization with the MRN complex in response to DNA damage it does not co-localize with the MRN complex on replicating chromatin. These data suggest that mMDC1 is a marker for both exogenously and endogenously generated DNA double-stranded breaks and that its interaction with the MRN complex is initiated exclusively by DNA damage. |
