| First Author | Stauffer JK | Year | 1990 |
| Journal | J Biol Chem | Volume | 265 |
| Issue | 20 | Pages | 11773-82 |
| PubMed ID | 2365699 | Mgi Jnum | J:10607 |
| Mgi Id | MGI:59055 | Doi | 10.1016/s0021-9258(19)38465-0 |
| Citation | Stauffer JK, et al. (1990) Nonconservative utilization of aldolase A alternative promoters. J Biol Chem 265(20):11773-82 |
| abstractText | Recently, analysis of the sequence and expression of the human aldolase A gene revealed the unique arrangement of three tandem promoters and exons preceding a common coding sequence. A muscle-specific promoter (M) and two flanking widely used promoters (N and H) produce mRNA species which, in their mature forms, differ only in the sequence of their 5'-untranslated regions. We have isolated and investigated the expression of a mouse aldolase A gene. This mouse gene represents a functional gene by sequence analysis, recombinational screening, and by transfection into C2C12 cells. Although there is a high degree of sequence similarity between the mouse and the human gene in the region of the alternative first exons, we have been unable to detect a functional utilization of the 5'-most promoter (N) in the mouse. Steady state mRNAs isolated from a variety of adult tissues and cultured cells were analyzed by RNase protection and primer extension to identify first exon utilization. Consistent with previous reports, exon M is found only in skeletal muscle and exon H, the housekeeping exon, is utilized in every tissue where aldolase A is expressed. Under identical conditions we fail to see any evidence of the N exon. Therefore, although sequence homology exists between rodents and primates in the N region, the absence of selective pressure to preserve its primate pattern of expression may have resulted in functional promoter extinction. |
