| First Author | Ouyang YB | Year | 2012 |
| Journal | Mitochondrion | Volume | 12 |
| Issue | 2 | Pages | 213-9 |
| PubMed ID | 21958558 | Mgi Jnum | J:192143 |
| Mgi Id | MGI:5464083 | Doi | 10.1016/j.mito.2011.09.001 |
| Citation | Ouyang YB, et al. (2012) miR-181 targets multiple Bcl-2 family members and influences apoptosis and mitochondrial function in astrocytes. Mitochondrion 12(2):213-9 |
| abstractText | Mitochondria are central to the execution of apoptosis, and the Bcl-2 protein family of pro- and anti-apoptotic proteins interacts with mitochondria to regulate apoptosis. Using bioinformatics we predicted that miR-181, a microRNA expressed in brain, could target the 3'UTRs of Bcl-2 family members Bcl-2-L11/Bim, Mcl-1, and Bcl-2. Using the luciferase reporter assay we confirmed these targets. We used mimic and inhibitor to alter miR-181a levels in primary astrocyte cultures and found miR-181a reduction was associated with increased Bcl-2 and Mcl-1 protein levels. Decreased miR-181a levels reduced glucose deprivation induced apoptosis, mitochondrial dysfunction, and loss of mitochondrial membrane potential in astrocytes. |
