| Primary Identifier | MGI:6101479 | Allele Type | Endonuclease-mediated |
| Attribute String | Null/knockout | Gene | H2-Ab1 |
| Inheritance Mode | Not Specified | Strain of Origin | NOD/ShiLtDvs-H2-K1<d-em1Dvs> H2-D1<b-em5Dvs>/Dvs |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Cas9 RNA and four guide sequences targeting Exon 2 (CCAACGGGACGCAGCGCATA, CGAAGCGCAGGTACTCCTCC, CGACGTGGGCGAGTACCGCG, ACACAACTACGAGGAGACGG) were injected into NOD/ShiLtDvs-H2-K1em1Dvs H2-D1em5Dvs/Dvs embryos yielding this 181bp deletion (ATACGGCTCGTGACCAGATACATCTACAACCGGGAGGAGTACCTGCGCTTCGACAGCGACGTGGGCGAGTACCGCGCGGT GACCGAGCTGGGGCGGCACTCAGCCGAGTACTACAATAAGCAGTACCTGGAGCGAACGCGGGCCGAGCTGGACACGGCGT GCAGACACAACTACGAGGAGA) within Exon 2 of H2-Ab1. Flow cytometry showed a paucity of CD8+ and CD4+ T-cells in the blood, and flow cytometry showing loss of H2-D, H2-K, and I-A on blood B-cells. |
