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Publication : Effects of congenital cataract mutation R116H on alphaA-crystallin structure, function and stability.

First Author  Pang M Year  2010
Journal  Biochim Biophys Acta Volume  1804
Issue  4 Pages  948-56
PubMed ID  20079887 Mgi Jnum  J:165084
Mgi Id  MGI:4836153 Doi  10.1016/j.bbapap.2010.01.001
Citation  Pang M, et al. (2010) Effects of congenital cataract mutation R116H on alphaA-crystallin structure, function and stability. Biochim Biophys Acta 1804(4):948-56
abstractText  alpha-crystallin is a molecular chaperone that maintains the optical properties of the lens and delays the onset scattering caused by aging-related protein aggregation. In this research, we found that the missense mutation R116H resulted in an altered size distribution, impaired packing of the secondary structures and modified quaternary structure with great hydrophobic exposure. The mutant exhibited a substrate-dependent chaperone (aggregation-inhibition) or anti-chaperone (aggregation-promotion) effect. Equilibrium unfolding experiments indicated that the mutation stabilized an aggregation-prone intermediate which was not populated during the unfolding of the wild-type protein. The accumulation of this intermediate greatly promoted the formation of non-native large oligomers or aggregates during unfolding. These results suggested that both the aggregation of the mutant upon stress and co-deposition with the target proteins were likely to be responsible for the onset of cataract.
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