| First Author | Xia D | Year | 2015 |
| Journal | Neuron | Volume | 85 |
| Issue | 5 | Pages | 967-81 |
| PubMed ID | 25741723 | Mgi Jnum | J:219929 |
| Mgi Id | MGI:5629994 | Doi | 10.1016/j.neuron.2015.02.010 |
| Citation | Xia D, et al. (2015) Presenilin-1 Knockin Mice Reveal Loss-of-Function Mechanism for Familial Alzheimer's Disease. Neuron 85(5):967-81 |
| abstractText | Presenilins play essential roles in memory formation, synaptic function, and neuronal survival. Mutations in the Presenilin-1 (PSEN1) gene are the major cause of familial Alzheimer's disease (FAD). How PSEN1 mutations cause FAD is unclear, and pathogenic mechanisms based on gain or loss of function have been proposed. Here, we generated Psen1 knockin (KI) mice carrying the FAD mutation L435F or C410Y. Remarkably, KI mice homozygous for either mutation recapitulate the phenotypes of Psen1(-/-) mice. Neither mutation altered Psen1 mRNA expression, but both abolished gamma-secretase activity. Heterozygosity for the KI mutation decreased production of Abeta40 and Abeta42, increased the Abeta42/Abeta40 ratio, and exacerbated Abeta deposition. Furthermore, the L435F mutation impairs hippocampal synaptic plasticity and memory and causes age-dependent neurodegeneration in the aging cerebral cortex. Collectively, our findings reveal that FAD mutations can cause complete loss of Presenilin-1 function in vivo, suggesting that clinical PSEN mutations produce FAD through a loss-of-function mechanism. |
