| First Author | Sander M | Year | 2000 |
| Journal | Development | Volume | 127 |
| Issue | 24 | Pages | 5533-40 |
| PubMed ID | 11076772 | Mgi Jnum | J:65594 |
| Mgi Id | MGI:1926842 | Doi | 10.1242/dev.127.24.5533 |
| Citation | Sander M, et al. (2000) Homeobox gene Nkx6.1 lies downstream of Nkx2.2 in the major pathway of beta-cell formation in the pancreas. Development 127(24):5533-40 |
| abstractText | Most insulin-producing beta-cells in the fetal mouse pancreas arise during the secondary transition, a wave of differentiation starting at embryonic day 13. Here, we show that disruption of homeobox gene Nkx6.1 in mice leads to loss of beta-cell precursors and blocks beta-cell neogenesis specifically during the secondary transition. In contrast, islet development in Nkx6. 1/Nkx2.2 double mutant embryos is identical to Nkx2.2 single mutant islet development: beta-cell precursors survive but fail to differentiate into beta-cells throughout development. Together, these experiments reveal two independently controlled pathways for beta-cell differentiation, and place Nkx6.1 downstream of Nkx2.2 in the major pathway of beta-cell differentiation. |
