| Primary Identifier | MGI:3702034 | Allele Type | Transgenic |
| Attribute String | Inducible, Recombinase | Gene | Tg(Igh-6-cre/ERT)30Afst |
| Strain of Origin | (C57BL/6 x CBA)F2 | Induced With | tamoxifen |
| Is Recombinase | true | Is Wild Type | false |
| description | Line 30 is a representative expressing line; line 24 was also created with a similar expression pattern. |
| molecularNote | The transgene was created by combining the Igh-6 enhancer with a minimal SV40 promoter to drive the expression of a cre-ERT mutant fusion protein. The fusion protein consisted of cre recombinase fused to the estrogen binding domain of the human estrogen receptor mutated at amino acid 521 (G521R). Expression in ~ 75% of peripheral blood B lymphocytes was confirmed with an anti-cre antibody. |
