| First Author | Ramkumar N | Year | 2016 |
| Journal | Nat Cell Biol | Volume | 18 |
| Issue | 12 | Pages | 1281-1291 |
| PubMed ID | 27870829 | Mgi Jnum | J:238551 |
| Mgi Id | MGI:5823085 | Doi | 10.1038/ncb3442 |
| Citation | Ramkumar N, et al. (2016) Crumbs2 promotes cell ingression during the epithelial-to-mesenchymal transition at gastrulation. Nat Cell Biol 18(12):1281-1291 |
| abstractText | During gastrulation of the mouse embryo, individual cells ingress in an apparently stochastic pattern during the epithelial-to-mesenchymal transition (EMT). Here we define a critical role of the apical protein Crumbs2 (CRB2) in the gastrulation EMT. Static and live imaging show that ingressing cells in Crumbs2 mutant embryos become trapped at the primitive streak, where they continue to express the epiblast transcription factor SOX2 and retain thin E-cadherin-containing connections to the epiblast surface that trap them at the streak. CRB2 is distributed in a complex anisotropic pattern on apical cell edges, and the level of CRB2 on a cell edge is inversely correlated with the level of myosin IIB. The data suggest that the distributions of CRB2 and myosin IIB define which cells will ingress, and we propose that cells with high apical CRB2 are basally extruded from the epiblast by neighbouring cells with high levels of apical myosin. |
