Primary Identifier | MGI:6363975 | Allele Type | Targeted |
Attribute String | Conditional ready | Gene | Gria3 |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A DNA fragment that contained a loxP sequence and pgk-1 promoter-driven neomycin phosphotransferase gene (Neo cassette) flanked by two Flp recognition target (frt) sites was inserted into a site 107 bp upstream of exon 11. The pgk-1 polyadenylation (poly-A) signal sequence was inserted downstream of the Neo cassette. The other loxP site was introduced into a site 113 bp downstream of exon 11 to eliminate the putative transmembrane domain after Cre-mediated recombination. |