Primary Identifier | MGI:5818274 | Allele Type | Targeted |
Attribute String | Inducible, Null/knockout, Recombinase | Gene | Tnnt2 |
Transmission | Germline | Strain of Origin | Not Specified |
Induced With | tamoxifen | Is Recombinase | true |
Is Wild Type | false |
molecularNote | A cassette encoding cre recombinase fused to 2 mutated murine estrogen receptor ligand-binding domains (Mer; Esr1*) followed by FRT flanked neo selection cassette are inserted into the start codon of Tnnt2 locus, with disruption of endogenous ATG by homologous recombination in ES cells. Flp-mediated recombination removed the selection cassette, leaving a single FRT site behind. |