| First Author | Ohno H | Year | 1995 |
| Journal | Science | Volume | 269 |
| Issue | 5232 | Pages | 1872-5 |
| PubMed ID | 7569928 | Mgi Jnum | J:28934 |
| Mgi Id | MGI:76471 | Doi | 10.1126/science.7569928 |
| Citation | Ohno H, et al. (1995) Interaction of tyrosine-based sorting signals with clathrin-associated proteins. Science 269(5232):1872-5 |
| abstractText | Tyrosine-based signals within the cytoplasmic domain of integral membrane proteins mediate clathrin-dependent protein sorting in the endocytic and secretory pathways. A yeast two-hybrid system was used to identify proteins that bind to tyrosine-based signals. The medium chains (mu 1 and mu 2) of two clathrin-associated protein complexes (AP-1 and AP-2, respectively) specifically interacted with tyrosine-based signals of several integral membrane proteins. The interaction was confirmed by in vitro binding assays. Thus, it is likely that the medium chains serve as signal-binding components of the clathrin-dependent sorting machinery. |
