| First Author | Jackson LL | Year | 1986 |
| Journal | Proc Natl Acad Sci U S A | Volume | 83 |
| Issue | 22 | Pages | 8496-500 |
| PubMed ID | 3464966 | Mgi Jnum | J:8464 |
| Mgi Id | MGI:56930 | Doi | 10.1073/pnas.83.22.8496 |
| Citation | Jackson LL, et al. (1986) Molecular cloning of mouse placental lactogen cDNA. Proc Natl Acad Sci U S A 83(22):8496-500 |
| abstractText | We have isolated a cDNA clone for the 23-kDa mouse placental lactogen II (mPL-II) from a phage lambda gt11 expression library containing cDNA synthesized from BALB/c placental RNA. Translation in vitro of placental mRNA selected by hybridization to the mPL-II cDNA clones yields a 26-kDa polypeptide that is the size of the expected precursor protein and that is immunoprecipitated with anti-mPL-II antiserum. The mPL-II cDNA clones hybridize to a 1.0-kilobase placental-specific mRNA. This mRNA, found in the fetal portion of the placenta, appears as early as day 10 of gestation and increases to a maximal level by day 12. The mPL-II cDNA nucleotide sequence has been determined. This sequence contains an open reading frame encoding a polypeptide of 222 amino acids with the amino-terminal 31 amino acids forming the signal sequence for secretion. The predicted secreted protein has 51% amino acid homology with mouse prolactin. |
