| First Author | Kuroyama H | Year | 2004 |
| Journal | Biochem Biophys Res Commun | Volume | 315 |
| Issue | 4 | Pages | 935-41 |
| PubMed ID | 14985102 | Mgi Jnum | J:88487 |
| Mgi Id | MGI:3033543 | Doi | 10.1016/j.bbrc.2004.01.127 |
| Citation | Kuroyama H, et al. (2004) Identification of a novel isoform of ZAP-70, truncated ZAP kinase. Biochem Biophys Res Commun 315(4):935-41 |
| abstractText | We identified a novel cDNA encoding truncated ZAP-70, which lacked the SH2 domain and a part of interdomain B, and named it truncated ZAP kinase (TZK). TZK was expressed in the thymus, spleen, and lymph nodes with ZAP-70. TZK was expressed in CD44+CD25- thymocytes up to mature T cells, but ZAP-70 was not expressed in CD44+CD25- or CD44+CD25+ thymocytes. ZAP-70 or TZK was transfected into P116 cells derived from a Jurkat T-cell line deficient in ZAP-70. The P116 cells with ZAP-70 induced the T-cell receptor-mediated signal transduction, but the cells expressing TZK did not. While ZAP-70 was accumulated at the immune synapse, TZK was not. Meanwhile, impaired phosphorylation of SLP-76, one of the substrates of ZAP-70, in P116 cells upon pervanadate stimulation was rescued in the cells expressing TZK. These findings show that TZK is a novel isoform of ZAP-70, which is expressed in pre-T-cell receptor-minus thymocytes and functions as a kinase not associated with T-cell receptor. |
