| First Author | Borggrefe T | Year | 1999 |
| Journal | Eur J Immunol | Volume | 29 |
| Issue | 6 | Pages | 1812-22 |
| PubMed ID | 10382743 | Mgi Jnum | J:56082 |
| Mgi Id | MGI:1340072 | Doi | 10.1002/(SICI)1521-4141(199906)29:06<1812::AID-IMMU1812>3.0.CO;2-J |
| Citation | Borggrefe T, et al. (1999) Cellular, intracellular, and developmental expression patterns of murine SWAP-70. Eur J Immunol 29(6):1812-22 |
| abstractText | SWAP-70 is part of a protein complex that catalyzes cell-free DNA recombination between immunoglobulin heavy chain gene switch region substrates. This report studies the expression pattern of SWAP-70 in mouse tissues, sorted cells, and cultured primary cells. SWAP-70 RNA is strongly increased upon switch-induction of spleen cells, and very weakly expressed in thymus and bone marrow. SWAP-70 protein is specifically expressed in B cells, and levels increase rapidly after stimulation. Tissue staining shows strong expression in germinal center B cells, while macrophages and T lymphocytes do not stain. SWAP-70 is not detected in early B cells in the bone marrow. Its expression during mouse ontogeny after birth correlates with the appearance of non-IgM isotypes. While SWAP-70 localizes to the cell nucleus in activated B cells, it is not tightly associated with the chromatin and is found in the cytoplasm as well. SWAP-70 expression is not increased by gamma or UV irradiation of spleen cells, nor does it depend on p53. These characteristics are consistent with the putative role of SWAP-70 in immunoglobulin class switching. |
