| First Author | Wang CC | Year | 2004 |
| Journal | Dev Cell | Volume | 7 |
| Issue | 3 | Pages | 359-71 |
| PubMed ID | 15363411 | Mgi Jnum | J:93035 |
| Mgi Id | MGI:3055633 | Doi | 10.1016/j.devcel.2004.08.002 |
| Citation | Wang CC, et al. (2004) A role of VAMP8/endobrevin in regulated exocytosis of pancreatic acinar cells. Dev Cell 7(3):359-71 |
| abstractText | Despite our general understanding that members of the SNARE superfamily participate in diverse intracellular docking/fusion events, the physiological role of the majority of SNAREs in the intact organism remains elusive. In this study, through targeted gene knockout in mice, we establish that VAMP8/endobrevin is a major player in regulated exocytosis of the exocrine pancreas. VAMP8 is enriched on the membrane of zymogen granules and exists in a complex with syntaxin 4 and SNAP-23. VAMP8-/- mice developed normally but showed severe defects in the pancreas. VAMP8 null acinar cells contained three times more zymogen granules than control acinar cells. Furthermore, secretagogue-stimulated secretion was abolished in pancreatic fragments derived from VAMP8-/- mice. In addition, VAMP8-/- mice were partially resistant to supramaximal caerulein-induced pancreatitis. These results suggest a major physiological role of VAMP8 in regulated exocytosis of pancreatic acinar cells by serving as a v-SNARE of zymogen granules. |
