| First Author | Komatsu M | Year | 2010 |
| Journal | Genes Cells | Volume | 15 |
| Issue | 2 | Pages | 91-100 |
| PubMed ID | 20002497 | Mgi Jnum | J:158538 |
| Mgi Id | MGI:4439006 | Doi | 10.1111/j.1365-2443.2009.01368.x |
| Citation | Komatsu M, et al. (2010) Multiple roles of PPARalpha in brown adipose tissue under constitutive and cold conditions. Genes Cells 15(2):91-100 |
| abstractText | Peroxisome proliferator-activated receptor alpha (PPARalpha) is a member of the nuclear receptor family, regulating fatty acid degradation in many organs. Two-dimensional SDS-PAGE of brown adipose tissue (BAT) from PPARalpha-null mice produced a higher-density spot. Proteomic analysis indicated that the protein was pyruvate dehydrogenase beta (PDHbeta). To observe PDHbeta regulation in BAT, the organ was stimulated by long-term cold exposure, and the activities of associated enzymes were investigated. Histological and biochemical analyses of BAT showed a significant decrease in the triglyceride content in wild-type mice and some degree of decrease in PPARalpha-null mice on cold exposure. Analyses of molecules related to glucose metabolism showed that the expression of PDHbeta is under PPARalpha-specific regulation, and that glucose degradation ability may decrease on cold exposure. In contrast, analyses of molecules related to fatty acid metabolism showed that numerous PPARalpha/gamma target molecules are induced on cold exposure, and that fatty acid degradation ability in wild-type mice is markedly enhanced and also increases to same degree in PPARalpha-null mice on cold exposure. Thus, this study proposes novel and multiple roles of PPARalpha in BAT. |
