Primary Identifier | MGI:6115471 | Allele Type | Targeted |
Attribute String | Not Specified | Gene | Pik3c3 |
Transmission | Germline | Strain of Origin | C57BL/6NTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT-flanked neomycin resistance cassette with a 5' loxP site was inserted upstream of exon 21. An F3-flanked puromycin resistance cassette with a 3' loxP site was inserted downstream of exon 21. Exon 22 was replaced with a modified exon in which a nucleotide substitution results in the amino acid substitution of alanine for aspartic acid at position 761 (D761A). Flp-mediated recombination removed both selection cassette. |