| First Author | Schweizer N | Year | 2021 |
| Journal | Nat Commun | Volume | 12 |
| Issue | 1 | Pages | 6042 |
| PubMed ID | 34654813 | Mgi Jnum | J:334667 |
| Mgi Id | MGI:6787574 | Doi | 10.1038/s41467-021-26252-5 |
| Citation | Schweizer N, et al. (2021) Sub-centrosomal mapping identifies augmin-gammaTuRC as part of a centriole-stabilizing scaffold. Nat Commun 12(1):6042 |
| abstractText | Centriole biogenesis and maintenance are crucial for cells to generate cilia and assemble centrosomes that function as microtubule organizing centers (MTOCs). Centriole biogenesis and MTOC function both require the microtubule nucleator gamma-tubulin ring complex (gammaTuRC). It is widely accepted that gammaTuRC nucleates microtubules from the pericentriolar material that is associated with the proximal part of centrioles. However, gammaTuRC also localizes more distally and in the centriole lumen, but the significance of these findings is unclear. Here we identify spatially and functionally distinct subpopulations of centrosomal gammaTuRC. Luminal localization is mediated by augmin, which is linked to the centriole inner scaffold through POC5. Disruption of luminal localization impairs centriole integrity and interferes with cilium assembly. Defective ciliogenesis is also observed in gammaTuRC mutant fibroblasts from a patient suffering from microcephaly with chorioretinopathy. These results identify a non-canonical role of augmin-gammaTuRC in the centriole lumen that is linked to human disease. |
