| First Author | Uematsu Y | Year | 1988 |
| Journal | Cell | Volume | 52 |
| Issue | 6 | Pages | 831-41 |
| PubMed ID | 3258191 | Mgi Jnum | J:93247 |
| Mgi Id | MGI:3056557 | Doi | 10.1016/0092-8674(88)90425-4 |
| Citation | Uematsu Y, et al. (1988) In transgenic mice the introduced functional T cell receptor beta gene prevents expression of endogenous beta genes. Cell 52(6):831-41 |
| abstractText | Transgenic mice were constructed with a functional T cell receptor beta gene. Transcription of the introduced gene is largely confined to T cells, but low levels of transcripts are also seen in B cells and in other tissues. Serological analyses show that most, if not all, of the T lymphocytes express the transgenic beta chain on the cell surface and lack beta chains encoded by endogenous beta genes. Molecular genetic analyses of uncloned and cloned T lymphocytes demonstrate that rearrangement of endogenous beta genes is incomplete. Partial D beta 1-J beta 1 rearrangements are found preferentially, while complete VDJ rearrangements are not seen. These findings show that expression of the transgene regulates the rearrangement of endogenous beta genes. Although the alpha beta T cell receptors of the transgenic mice are homogeneous with respect to the beta chain, they are fully functional, at least in a variety of allogeneic responses. |
