| First Author | Dominov JA | Year | 1996 |
| Journal | Dev Genet | Volume | 19 |
| Issue | 2 | Pages | 108-18 |
| PubMed ID | 8900043 | Mgi Jnum | J:36227 |
| Mgi Id | MGI:83666 | Doi | 10.1002/(SICI)1520-6408(1996)19:2<108::AID-DVG2>3.0.CO;2-D |
| Citation | Dominov JA, et al. (1996) POU homeodomain genes and myogenesis. Dev Genet 19(2):108-18 |
| abstractText | We show that members of the POU homeodomain family are among the transcription factors expressed in developing mouse skeletal muscle. From a cDNA library prepared from fetal muscle mRNA, we cloned a cDNA identical to that of Brn-4, a POU class ill gene previously cloned from neural tissues. In limb muscle, we found that Brn-4 mRNA expression was highest at embryonic days 15-18, declined after birth, and was undetectable in adults. The mRNAs of two additional POU genes, Emb (POU class VI) and Oct-1 (POU class II), were also expressed in developing muscle and, unlike Brn-4, continued to be expressed in postnatal and adult muscles. In skeletal muscle, expression of Brn-4 is myogenin-dependent, because muscles from myogenin- deficient fetuses contained much less Brn-4 mRNA than muscles from myogenin-expressing littermates. In contrast, expression of Emb was the same in the presence or absence of myogenin. The distinct pattern of Brn-4 mRNA expression and its dependence on a myogenic regulatory factor suggest that Brn-4 is part of the network of interacting transcription factors that control muscle-specific gene expression during mammalian myogenesis. (C) 1996 Wiley- Liss, Inc. |
