| Primary Identifier | MGI:6158302 | Allele Type | Targeted |
| Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Col1a1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| description | Parental ES cells were derived from FVB.129P2-Trp53tm1Brn Rb1tm2Brn mice and containing in addition to the targeted Col1a1tm2Nki allele. |
| molecularNote | RMCE retargeted Col1a1tm2Nki to replace the frt-flanked PGK-Neo cassette in the 3' UTR of the Col1a1 by a "Flp-in" plasmid encoding a pFrt-invCAG-Nfib_v3-Luc inversion transgene, called inv-Cag-Luc2, by co-transfected with a Flpe-expressing plasmid to facilitate recombination into the 3' UTR of the Col1a1 locus. This transgene contains the constitutive CAGGs promoter that drives the expression of the Hygromycin-resistance gene, present in the targeted Col1a1 locus. Upon expression of the Cre recombinase, the promoter is inverted due to flanking mutant loxP sites, i.e. lox66 and lox71, placing the promoter in front of the firefly Luciferase2 gene and the cDNA for the short variant lacking exon 9-11 (variant 3) of Nfib. |
