| Primary Identifier | MGI:3760543 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rapgef6 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = EB3, derived from E14TG2a. EB3 cells carry one allele of Pou5f1 that has been inactivated via targeted integration of E14TG2a ES cells with an IRESBSDpA selection cassette. J:126164 does not mention whether the null allele of Pou5f1 is also present in the line of mice created. Phenotypic Similarity to Human Syndrome: Oligoasthenoteratozoospermia (OAT) Syndrome J:218581 |
| molecularNote | Exon 21 was removed by cre-mediated deletion following a cross to Tg(ACTB-cre)13Miya mice to create an out of frame deletion. Absence of protein product was confirmed by western blot on protein extracts from lung, thymus, brain, spleen and pancreas. |
