| First Author | Reeves JE | Year | 1995 |
| Journal | Mol Membr Biol | Volume | 12 |
| Issue | 2 | Pages | 201-8 |
| PubMed ID | 7540914 | Mgi Jnum | J:28355 |
| Mgi Id | MGI:75973 | Doi | 10.3109/09687689509027508 |
| Citation | Reeves JE, et al. (1995) The surf-4 gene encodes a novel 30 kDa integral membrane protein. Mol Membr Biol 12(2):201-8 |
| abstractText | The mouse surfeit locus is a tight cluster of at least six genes (surf-1 to -6), unrelated by sequence homology, whose unique organization is conserved in vertebrates. We show that the surf-4 coding sequence is conserved between mouse and human. Primary sequence analysis predicts that the mouse surf-4 protein contains seven transmembrane domains and a double lysine endoplasmic reticulum (ER) retrieval motif on the carboxyl terminus. Translation of the mouse surf-4 cDNA in vitro resulted in the production of a 30 kDa membrane protein. Salt and detergent extraction procedures showed that the surf-4 protein associated tightly with the microsomal membranes. Proteolysis protection of 14 and 3 kDa fragments indicates that the surf-4 protein contains at least two membrane spanning domains: this is consistent with the proposed topology. Addition of the c-Myc epitope into three different regions of the surf-4 protein resulted in transfectants that expressed a myc-tagged protein. Immunofluorescence analysis of the three surf-4 myc chimeras yielded a cytoplasmic staining pattern. Consistent with the presence of the ER retrieval motif, the surf-4 myc protein was not detected at the plasma membrane. A model for the proposed structure of the surf-4 protein is presented. |
