| First Author | Gotoh M | Year | 1998 |
| Journal | Jpn J Cancer Res | Volume | 89 |
| Issue | 8 | Pages | 849-54 |
| PubMed ID | 9765622 | Mgi Jnum | J:49838 |
| Mgi Id | MGI:1289115 | Doi | 10.1111/j.1349-7006.1998.tb00639.x |
| Citation | Gotoh M, et al. (1998) Sequence analysis of genes encoding rodent homologues of the human tumor-rejection antigen SART-1. Jpn J Cancer Res 89(8):849-54 |
| abstractText | Human SART-1 (hSART-1) gene encodes a 125 kD protein with a leucine- zipper motif expressed in the nucleus of all proliferating cells, and a 43 kD protein expressed in the cytosol of most epithelial cancers. In this study, two rodent genes (rSART-1 and mSART-1) homologous to hSART- 1 were cloned from cDNA libraries of murine brain and a rat tumor cell line, respectively. mSART-1 and rSART-1 were highly homologous to hSART- 1 with 86% and 84% identity at the nucleotide level, and 95% and 91% at the protein level, respectively. The leucine zipper domain and two basic amino acid portions that bind DNA, as well as peptide sequences recognized by human cytotoxic T lymphocytes (CTLs), were all conserved in these rodent genes. Nuclear protein homologous to the 125 kD hSART- 1(800) protein, but not to the 43 kD cytosol SART-1(259) protein, was detectable with specific antibody in the nuclear fractions of rodent tumor cell lines, and normal rodent fetal liver and testis. These rodent genes should be a novel tool for studies on the biological roles of the SART-1 gene, and also in the construction of animal models of specific immunotherapy using SART-1 gene products. |
