Primary Identifier | MGI:6307097 | Allele Type | Endonuclease-mediated |
Attribute String | Epitope tag | Gene | Nxph4 |
Strain of Origin | C57BL/6J | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 genome editing is used to insert a 3xFLAG tag sequence at the end of the third domain of the gene (after 5'-CTCCAAGCGTGTGGAGTTC). The PAM site (AGG) was also mutated to AGA to avoid cleaving by Cas9. |