Primary Identifier | MGI:6099123 | Allele Type | Targeted |
Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Gt(ROSA)26Sor |
Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A construct comprising the following elements was inserted into the host gene: a loxP site flanked STOP cassette, improved estrogen receptor nuclear translocation domain sequence fused to the cDNA for the fungal I-PpoI homing endonuclease, followed by an IRES and the EGFP fluorescent reporter gene. This creates a conditional-ready allele that will only be expressed after cre-mediated deletion of the STOP cassette and only after application of tamoxifen will the expressed endonuclease be transported to the nucleus where it targets DNA. |