| Primary Identifier | MGI:2155982 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Plcg1 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | Injection of mutant ES cells into wild-type (C57BL/6) blastocysts results in chimeric animals with distended abdomens, some of which display premature death at 3 months postpartum. Extensive chimerism (>70% agouti coat color) results in lethality. Chimeras with a 30-70% agouti coat color display: enlarged, polycystic kidneys, renal dysplasia (at 2 weeks), dilated renal tubules (at >2 weeks), and occasional glomerulosclerosis in high % chimeras with severe renal dysplasia. Mutant ES cells fail to contribute to lymphoid and hematopoietic organs (including thymus, spleen, bone marrow and red blood cells) in chimeric mice (J:73254). |
| molecularNote | A PGK-neomycin resistance cassette replaced approximately 17kb of sequence encoding the functional domains. |
